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The details of isolation, purification, and structural elucidation of unknown-1 (mitragynine pseudoindoxyl) and its chemical synthesis are described in the Supporting Information

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Time domain diffuse correlation spectroscopy with a high coherence pulsed source: In vivo and phantom results

For the induction of recombinant proteins, the yeast cells grown in SD minimal medium without uracil at 28C for 1 days were transferred to 10 volumes of uracil-less SD medium containing 2% (w/v) galactose instead of glucose to activate the GAL1 promoter on pYES2, and cultured at 28C for 1 days